Composite

Part:BBa_K338004

Designed by: Lucas Hartsough   Group: iGEM10_Caltech   (2010-10-26)

PHA Synthase Composite, Part 2/2

This is half of a planned part which would contain all three PHA synthase genes required to produce [http://en.wikipedia.org/wiki/Polyhydroxybutyrate polyhydroxybutyrate] (PHB) in cells: phaA, phaB1, phaC1. This half contains phaB1 and phaC1: BBa_K156013 & BBa_K156014. It was designed to be ligated downstream of part BBa_K338003.

Usage and Biology

Although not a finished product, it was tested in DH5α cells grown in 5mM glucose for 48 hours (5mL LB culture) at 37°C. The cells were then imaged at 100x, producing the following figures:

Caltech10Plastic1.jpg

Caltech10Plastic2.jpg

The small inclusion bodies suggest the production of an unknown, but possibly plastic-related, substance. A similar construct, pSYL105, caused the formation of large inclusion bodies in cells that produced plastic, lending credence to this possibility. [1]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1348
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 697
    Illegal AgeI site found at 1047
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/biosynthesis/plastic
Parameters
None